keywords: Antimicrobial activities, Mushroom, Phytonutrient analysis.
The phytochemical, mineral and nutrient compositions of the two medicinal mushrooms: Ganoderma lucidum and Pleurotus tuberregium as well as its antimicrobial activity were studied. Phytochemical analysis of Ganoderma lucidum and Pleurotus tuberregium mushrooms yielded (µg/m) saponin (20.462; 2.603), flavonoid (25.000; 5.000), phenolic (38.500; 20.400), tannin (23.091; 6.8107), and alkaloid (0.360%; 0.220%)espectively. Their proximate analysis gave high moisture content of the both mushrooms (Ganoderma lucidum = 83.200±0.400% and Pleurotus tuberregium = 87.288±0.84%). Other proximate analysis (%) recorded for Ganoderma lucidum and Pleurotus tuberregium mushrooms include: ash content (8.352±0.4; 14.225±0.8), crude protein (20.588±0.8; 21.20±0.7), crude fibre (4.103±0.5; 3.499±0.3), carborhydrate (63.906; 58.621). while the yielded fat content were (3.051±0.2 % 2.455±0.8%) and vitamin c were (1550.998; 623.300 µg/ml) respectively. However mineral composition analysis yielded (g/kg) the following: potassium (92.300; 83.000), nitrogen (3.294; 3.392), magnesium (2.320; 2.460), iron (1.400; 1.900), sodium (0.800; 0.500), manganese (0.610; 0.162), and zinc (0.490; 0.690) respectively. The antimicrobial analysis of these mushroom proved the susceptibility effect of the ethanol extract of the two mushrooms against the selected bacteria and fungi. For Ganoderma lucidum the minimum inhibitory zone for ethanol extracts against Staphylococccus aureus was at 3.125% concentration however better zone of inhibition was recorded at 6.25% concentration while Pleurotus tuberregium ethanol extract against Staphylococccus aureus shows that increase in concentration increases the zone of inhibition. However, Bacillus sp. was recorded to be resistant against the lower concentration of both extracts (3.125% - 12.500%)